PA5-87549

antibody from Invitrogen Antibodies

Targeting: SNAP25 bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25

Western blot

Antibody data

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Validation data

Product number: PA5-87549 - Provider product page
Provider: Invitrogen Antibodies
Product name: SNAP25 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG Positive Samples: HeLa, SH-SY5Y, PC-12; Cellular Location: Cell junction, Cell membrane, Cytoplasm, Lipid-anchor, perinuclear region, synapse, synaptosome
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.99 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

SNAP25 protein structure - PA5-87549 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SNAP25 in paraffin-embedded rat brain. Samples were incubated with SNAP25 Polyclonal antibody (Product # PA5-87549) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SNAP25 in paraffin-embedded human brain. Samples were incubated with SNAP25 Polyclonal antibody (Product # PA5-87549) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of SNAP25 in paraffin-embedded mouse brain. Samples were incubated with SNAP25 Polyclonal antibody (Product # PA5-87549) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Immunofluorescence) analysis of SNAP25 in rat brain. Samples were incubated with SNAP25 Polyclonal antibody (Product # PA5-87549) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Immunofluorescence) analysis of SNAP25 in mouse brain. Samples were incubated with SNAP25 Polyclonal antibody (Product # PA5-87549) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.