NBP1-04344

antibody from Novus Biologicals

Targeting: SNAP25 bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-04344

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-04344, RRID:AB_1522413

Product name

Mouse Monoclonal SNAP25 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).