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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MA5-38005 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC61alpha Recombinant Rabbit Monoclonal Antibody (ARC2323)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: 293T, SH-SY5Y, Mouse lung, Mouse testis, Mouse brain, Rat testis Immunogen sequence: SMGAIFEDPV HVVVYIIFML GSCAFFSKTW IEVSGSSAKD VAKQLKEQQM VMRGHRDTSM VHELNRYIPT AAAFGGLCIG ALSVLADFLG AIGSGTGILL A
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- ARC2323
- Vial size
- 100 µL
- Concentration
- 1.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of SEC61alpha using SEC61alpha Recombinant Monoclonal Antibody (Product # MA5-38005) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
