Explore
Validate
Learn
Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- HPA007533 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA007533, RRID:AB_2667855
- Product name
- Anti-NFIX
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NFIX, Gene description: nuclear factor I/X (CCAAT-binding transcription factor), Alternative Gene Names: NF1A, Validated applications: WB, IHC, ICC, ChIP, Uniprot ID: , Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references MiR-744-5p inducing cell death by directly targeting HNRNPC and NFIX in ovarian cancer cells.
Kleemann M, Schneider H, Unger K, Sander P, Schneider EM, Fischer-Posovszky P, Handrick R, Otte K
Scientific reports 2018 Jun 13;8(1):9020
Scientific reports 2018 Jun 13;8(1):9020
No comments: Submit comment
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-NFIX (HPA007533, Lot 000050733) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
