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LearnPA5-41495
antibody from Invitrogen Antibodies
Targeting: HCFC1
CFF, HCF-1, HCF1, HFC1, MGC70925, MRX3, PPP1R89, VCAF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-41495 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCFC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: GTRLLVFGGM VEYGKYSNDL YELQASRWEW KRLKAKTPKN GPPPCPRLGH Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of transformed bacteria cell lysate using an anti-HCFC1 polyclonal antibody (Product # PA5-41495). Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit AlexaFluor 680; Secondary Antibody Dilution: 1:10000. LANE 1) HIS-HCFC1 16-363aa (42kD) transformed bacteria lysate; LANE 2) GFP-HCFC1 363-2002aa (73kD) transformed bacteria lysate elution sample.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cell lysate using an anti-HCFC1 polyclonal antibody (Product # PA5-41495).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous HCFC1 protein using Anti-HCFC1 Antibody: ChIP was performed using Anti-HCFC1 Polyclonal Antibody (Product # PA5-41495, 2.5 µg) on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to RBL1 and CDC25A transcription start sites and SAT alpha satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
