NB100-68209

antibody from Novus Biologicals

Targeting: HCFC1 CFF, HCF-1, HCF1, HFC1, MGC70925, MRX3, PPP1R89, VCAF

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-68209

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-68209, RRID:AB_1108786

Product name

Rabbit Polyclonal Host Cell Factor 1/HCFC1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Interaction of DNA demethylase and histone methyltransferase upregulates Nrf2 in 5-fluorouracil-resistant colon cancer cells.

Kang KA, Piao MJ, Ryu YS, Kang HK, Chang WY, Keum YS, Hyun JW
Oncotarget 2016 Jun 28;7(26):40594-40620

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A transcriptional regulatory role of the THAP11-HCF-1 complex in colon cancer cell function.

Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D
Molecular and cellular biology 2012 May;32(9):1654-70

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The evolutionarily conserved longevity determinants HCF-1 and SIR-2.1/SIRT1 collaborate to regulate DAF-16/FOXO.

Rizki G, Iwata TN, Li J, Riedel CG, Picard CL, Jan M, Murphy CT, Lee SS
PLoS genetics 2011 Sep;7(9):e1002235

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).