PA5-30222

antibody from Invitrogen Antibodies

Targeting: HCFC1 CFF, HCF-1, HCF1, HFC1, MGC70925, MRX3, PPP1R89, VCAF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30222

Provider

Invitrogen Antibodies

Product name

HCFC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, A549, A549 nuclear extract, rat brain.

Concentration

0.76 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HCF1 using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a HCF1 polyclonal antibody (Product # PA5-30222) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HCFC1 was performed by separating 30 µg of A549 whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HCFC1 Polyclonal Antibody (Product # PA5-30222) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HCFC1 Polyclonal Antibody (Product # PA5-30222). Sample (30 µg of whole cell lysate). Lane A: HeLa. 5% SDS PAGE. HCFC1 Polyclonal Antibody (Product # PA5-30222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HCFC1 was performed by separating 50 µg of rat tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HCFC1 Polyclonal Antibody (Product # PA5-30222) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.