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LearnPA5-30222
antibody from Invitrogen Antibodies
Targeting: HCFC1
CFF, HCF-1, HCF1, HFC1, MGC70925, MRX3, PPP1R89, VCAF
Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- PA5-30222 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCFC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, A549, A549 nuclear extract, rat brain. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.76 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HCF1 using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a HCF1 polyclonal antibody (Product # PA5-30222) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HCFC1 was performed by separating 30 µg of A549 whole cell and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HCFC1 Polyclonal Antibody (Product # PA5-30222) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HCFC1 Polyclonal Antibody (Product # PA5-30222). Sample (30 µg of whole cell lysate). Lane A: HeLa. 5% SDS PAGE. HCFC1 Polyclonal Antibody (Product # PA5-30222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HCFC1 was performed by separating 50 µg of rat tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HCFC1 Polyclonal Antibody (Product # PA5-30222) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
