PA5-34756

antibody from Invitrogen Antibodies

Targeting: GRIN2B GluN2B, NMDAR2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34756

Provider

Invitrogen Antibodies

Product name

NMDAR2B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, mouse brain, rat brain.

Concentration

0.79 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NMDA Receptor 2B using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a NMDA Receptor 2B polyclonal antibody (Product # PA5-34756) at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NMDAR2B was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NMDAR2B Polyclonal Antibody (Product # PA5-34756) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NMDAR2B Polyclonal Antibody (Product # PA5-34756). Sample (30 µg of whole cell lysate). A: Hep G2.5% SDS PAGE. NMDAR2B Polyclonal Antibody (Product # PA5-34756) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of NMDAR2B was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR2B Polyclonal Antibody (Product # PA5-34756) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.