Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-34756 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NMDAR2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, mouse brain, rat brain. Predicted reactivity: Mouse (97%), Rat (96%), Dog (97%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.79 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NMDA Receptor 2B using 50 µg of mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a NMDA Receptor 2B polyclonal antibody (Product # PA5-34756) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NMDAR2B was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a NMDAR2B Polyclonal Antibody (Product # PA5-34756) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NMDAR2B Polyclonal Antibody (Product # PA5-34756). Sample (30 µg of whole cell lysate). A: Hep G2.5% SDS PAGE. NMDAR2B Polyclonal Antibody (Product # PA5-34756) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NMDAR2B was performed in DIV14 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR2B Polyclonal Antibody (Product # PA5-34756) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.