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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- GTX31086 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX31086, RRID:AB_10719632
- Product name
- NMDAR2B (phospho Tyr1472) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Zebrafish
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western Blot: NMDA receptor 2B [Tyr1472] Antibody [GTX31086] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot: NMDA NR2B Phosphospecific Antibody [GTX31086] - Western Blot analysis of NMDA NR2B in rat tissue lysate, using GTX31086. Immunolabeling of the 180 kDa NR2B protein is blocked by the phosphopeptide, but not by non-phosphorylated peptide.
