H00002904-M01

antibody from Abnova Corporation

Targeting: GRIN2B GluN2B, NMDAR2B

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002904-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002904-M01, RRID:AB_1111876

Product name

GRIN2B monoclonal antibody (M01), clone 2G5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant GRIN2B.

Antigen sequence

HGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIM
EEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVG
WELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLY
CTKEE


Isotype

IgG

Antibody clone number

2G5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GRIN2B is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CAPN1 and GRIN2B. HeLa cells were stained with anti-CAPN1 rabbit purified polyclonal 1:1200 and anti-GRIN2B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)