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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- ABIN152455 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) antibody
- Antibody type
- Polyclonal
- Antigen
- ABIN152455 was raised against a c-terminal fusion protein representing amino acids1253 - 1391 of rat NMDAR2B.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 10 µg
- Concentration
- 0.2 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Decrease in N-methyl-D-aspartic acid receptor-NR2B subunit levels by intrathecal short-hairpin RNA blocks group I metabotropic glutamate receptor-mediated hyperalgesia.
Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants.
Dual neuroprotective signaling mediated by downregulating two distinct phosphatase activities of PTEN.
Gonadotropin-releasing hormone neurons, NMDA receptors, and their regulation by steroid hormones across the reproductive life cycle.
Developmental and regional expression in the rat brain and functional properties of four NMDA receptors.
Ligand affinities at recombinant N-methyl-D-aspartate receptors depend on subunit composition.
Heteromeric NMDA receptors: molecular and functional distinction of subtypes.
Molecular cloning and characterization of the rat NMDA receptor.
Gabra BH, Kessler FK, Ritter JK, Dewey WL, Smith FL
The Journal of pharmacology and experimental therapeutics 2007 Jul;322(1):186-94
The Journal of pharmacology and experimental therapeutics 2007 Jul;322(1):186-94
Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants.
Bai JY, Zeng L, Hu YL, Li YF, Lin ZP, Shang SC, Shi YS
Biotechnology letters 2007 Dec;29(12):2007-12
Biotechnology letters 2007 Dec;29(12):2007-12
Dual neuroprotective signaling mediated by downregulating two distinct phosphatase activities of PTEN.
Ning K, Pei L, Liao M, Liu B, Zhang Y, Jiang W, Mielke JG, Li L, Chen Y, El-Hayek YH, Fehlings MG, Zhang X, Liu F, Eubanks J, Wan Q
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Apr 21;24(16):4052-60
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Apr 21;24(16):4052-60
Gonadotropin-releasing hormone neurons, NMDA receptors, and their regulation by steroid hormones across the reproductive life cycle.
Gore AC
Brain research. Brain research reviews 2001 Nov;37(1-3):235-48
Brain research. Brain research reviews 2001 Nov;37(1-3):235-48
Developmental and regional expression in the rat brain and functional properties of four NMDA receptors.
Monyer H, Burnashev N, Laurie DJ, Sakmann B, Seeburg PH
Neuron 1994 Mar;12(3):529-40
Neuron 1994 Mar;12(3):529-40
Ligand affinities at recombinant N-methyl-D-aspartate receptors depend on subunit composition.
Laurie DJ, Seeburg PH
European journal of pharmacology 1994 Aug 16;268(3):335-45
European journal of pharmacology 1994 Aug 16;268(3):335-45
Heteromeric NMDA receptors: molecular and functional distinction of subtypes.
Monyer H, Sprengel R, Schoepfer R, Herb A, Higuchi M, Lomeli H, Burnashev N, Sakmann B, Seeburg PH
Science (New York, N.Y.) 1992 May 22;256(5060):1217-21
Science (New York, N.Y.) 1992 May 22;256(5060):1217-21
Molecular cloning and characterization of the rat NMDA receptor.
Moriyoshi K, Masu M, Ishii T, Shigemoto R, Mizuno N, Nakanishi S
Nature 1991 Nov 7;354(6348):31-7
Nature 1991 Nov 7;354(6348):31-7
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- NR2B in rat hippocampus stained with ABIN152455.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Lane 1:20 ug of rat brain hippocampus was loaded and ABIN152455 labels a band at 180 kDa. Lanes 2-3: HEK 293 cells expressing NR2A or NR2B respectively. The antibody labels only NR2B and shows no reactivity with NR2A. Non-specific staining of additional bands is seen in HEK293 cells but not in rat brain.
