21920-1-AP

antibody from Invitrogen Antibodies

Targeting: GRIN2B GluN2B, NMDAR2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21920-1-AP

Provider

Invitrogen Antibodies

Product name

GRIN2B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: YLDQFRTKE NSPHWEHVDL TDIYKERSDD FKRDSVSGGG PCTNRSHIKH GTGDKHGVVS GVPAPWEKNL TNVEWEDRSG GNFCRSCPSK LHNYSTTVTG QNSGRQACIR CEACKKAGNL YDISEDNSLQ ELDQPAAPVA VTSNASTTKY PQSPTNSKAQ KKNRNKLRRQ HSYDTFVDLQ KEEAALAPRS VSLKDKGRFM DGSPYAHMFE MSAGESTFAN NKSSVPTAGH HHHNNPGGGY MLSKSLYPDR VTQNPFIPTF GDDQCLLHGS KSYFFRQPTV AGASKARPDF RALVTNKPVV SALHGAVPAR FQKDICIGNQ SNPCVPNNKN PRAFNGSSNG HVYEKLSSIE SDV (1133-1484 aa encoded by BC113620)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.38 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NR2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (GRIN2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SH-SY5Y cells using 21920-1-AP (NR2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 21920-1-AP (NR2B antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 21920-1-AP (NR2B antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 SH-SY5Y cells were stained with 0.2ug NR2B antibody (21920-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NR2B (IP:21920-1-AP, 3ug; Detection:21920-1-AP 1:2000) with mouse brain tissue lysate 6000ug.