14-9790-82

antibody from Invitrogen Antibodies
Targeting: SPIB SPI-B
Provider product page for 14-9790-82
Western blot
Immunohistochemistry

Antibody data

Product number
14-9790-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
SPI-B Monoclonal Antibody (235D), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 235D monoclonal antibody recognizes human SPI-B. SPI-B is a member of the ETS transcription factor family which is comprised of DNA binding proteins that function in lymphoid development and activity. SPI-B binds the PU box, a purine-rich DNA sequence (5'-GAGGAA-3'). SPI-B is expressed in mature B cells, immature T cells, plasmacytoid dendritic cells, and microfold cells (M cells) of the intestine. In human tonsil tissue, high levels of SPI-B are expressed in plasmacytoid dendritic cells, while lower levels of expression are found in germinal center B cells. Applications Reported: This 235D antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 235D antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
235D
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
SPIB protein structure - 14-9790-82 shown in red.