Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- 14-9790-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPI-B Monoclonal Antibody (235D), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 235D monoclonal antibody recognizes human SPI-B. SPI-B is a member of the ETS transcription factor family which is comprised of DNA binding proteins that function in lymphoid development and activity. SPI-B binds the PU box, a purine-rich DNA sequence (5'-GAGGAA-3'). SPI-B is expressed in mature B cells, immature T cells, plasmacytoid dendritic cells, and microfold cells (M cells) of the intestine. In human tonsil tissue, high levels of SPI-B are expressed in plasmacytoid dendritic cells, while lower levels of expression are found in germinal center B cells. Applications Reported: This 235D antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This 235D antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 235D
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references SPIB, a novel immunohistochemical marker for human blastic plasmacytoid dendritic cell neoplasms: characterization of its expression in major hematolymphoid neoplasms.
The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells.
The Ets protein Spi-B is expressed exclusively in B cells and T cells during development.
Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1.
Montes-Moreno S, Ramos-Medina R, Martínez-López A, Barrionuevo Cornejo C, Parra Cubillos A, Quintana-Truyenque S, Rodriguez Pinilla SM, Pajares R, Sanchez-Verde L, Martinez-Torrecuadrada J, Roncador G, Piris MA
Blood 2013 Jan 24;121(4):643-7
Blood 2013 Jan 24;121(4):643-7
The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells.
Kanaya T, Hase K, Takahashi D, Fukuda S, Hoshino K, Sasaki I, Hemmi H, Knoop KA, Kumar N, Sato M, Katsuno T, Yokosuka O, Toyooka K, Nakai K, Sakamoto A, Kitahara Y, Jinnohara T, McSorley SJ, Kaisho T, Williams IR, Ohno H
Nature immunology 2012 Jun 17;13(8):729-36
Nature immunology 2012 Jun 17;13(8):729-36
The Ets protein Spi-B is expressed exclusively in B cells and T cells during development.
Su GH, Ip HS, Cobb BS, Lu MM, Chen HM, Simon MC
The Journal of experimental medicine 1996 Jul 1;184(1):203-14
The Journal of experimental medicine 1996 Jul 1;184(1):203-14
Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1.
Ray D, Bosselut R, Ghysdael J, Mattei MG, Tavitian A, Moreau-Gachelin F
Molecular and cellular biology 1992 Oct;12(10):4297-304
Molecular and cellular biology 1992 Oct;12(10):4297-304
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 5 µg/mL of Anti-Human SPI-B Purified (right) followed by Anti-Mouse Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.