Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 11-8052-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Spi-B Antibody
- Antibody type
- Polyclonal
- Description
- Spi-B belongs to the ETS family transcription containing a conserved ETS domain which specifically binds to a purine-rich DNA sequence called the PU-box (5'-GAGGAA-3'). Spi-B has two known isoforms (28 and 18 kDa) localized in nucleus and cytoplasm. Spi-B contains two transactivation domains (TAD) at the N- terminal end which may be required for transcriptional activation of the target genes. Spi-B is involved in development of plasmacytoid dendritic cells (natural interferon producing cells) and B-cell development. Spi-B acts as a lymphoid specific enhancer. Spi-B is highly expressed in plasmacytoid dendritic cells (pDCs), B-cells and stem cell populations of the liver.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
A partial length recombinant Spi-B
protein (amino acids 50-260) was us
ed as the immunogen for this antibo
dy.- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freezing and thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Spi-B. Anti-Spi-B antibody (11-8052) was used at 4 µg/ml on K562 lysate.
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Spi-B . Anti- Spi-B antibody (11-8052) in human Colon tissue at 5 µg/ml.