MA1-10329

antibody from Invitrogen Antibodies

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-10329

Provider

Invitrogen Antibodies

Product name

Phospho-EGFR (Tyr1173) Monoclonal Antibody (EM-13)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

EM-13

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 (Product # MA1-10329) in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 (Product # MA1-10329) in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY1173) (EM-13) purified Monoclonal antibody (Product # MA1-10329) (concentration in sample 3 µg/mL, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (Product # MA1-10329) (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (Product # MA1-10329) (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.