MA1-24678

antibody from Invitrogen Antibodies

Targeting: EGFR ERBB, ERBB1

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-24678

Provider

Invitrogen Antibodies

Product name

EGFR Monoclonal Antibody (29.1)

Antibody type

Monoclonal

Antigen

Other

Description

Recommended positive controls: A431, rat ovary.

Antibody clone number

29.1

Concentration

8.9 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Mouse Monoclonal Antibody (Product # MA1-24678) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.