GTX25650

antibody from GeneTex

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX25650

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25650, RRID:AB_380623

Product name

EGFR (phospho Tyr1086) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of A431 cells unstimulated (1) or stimulated with 200 ng/mL EGF for 15 minutes (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4¢XC, then incubated with the EGFR [pY1086] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphotyrosine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and signals were detected. The data show that only the phosphopeptide corresponding to EGFR [pY1086] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of EGFR [pY1086] phosphorylation by the addition of EGF to this cell system.