Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX42893 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX42893, RRID:AB_11173335
- Product name
- EGFR antibody [13G8]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- Upon receipt, store at °V20°C. Avoid multiple freeze/thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Detection of endogenous EGFR. Whole cell lysates of serum starved tumor cells (20,000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 0.5 μg/mL GTX42893 for 1 hour at RT and developed by ECL (exp. time: 30 sec).x000D Lane 1: A431 x000D Lane 2: A549 x000D Lane 3: SKOV3 x000D Lane 4: OVCAR5 x000D Lane 5: HaCaT x000D Lane 6: PC3 x000D Lane 7: HeLa x000D Lane 8: HepG2