Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX42907 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX42907, RRID:AB_11173138
- Product name
- EGFR (phospho Tyr1045) antibody [11C2]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- EGFR Activation. Serum starved MDA-MB 468 cells were incubated with 10ng/ml EGF for the indicated times. Whole cell lysates were prepared and separated by SDS-PAGE (20,000 cells/lane). The blot was probed with 0.5μg/ml GTX42907 for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1 hour EGF Lane 6: 2 hour EGF Lane 7: 4 hour EGF Lane 8: 8 hour EGF