UM500070

antibody from OriGene

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

UM500070

Provider

OriGene

Proper citation

OriGene Cat#UM500070, RRID:AB_2629083

Product name

EGFR mouse monoclonal antibody,clone UMAB95

Antibody type

Monoclonal

Description

EGFR mouse monoclonal antibody,clone UMAB95

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

EGFR

Isotype

IgG

Antibody clone number

UMAB95

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EGFR monoclonal antibody (Clone UMAB95).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:250.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-EGFR mouse monoclonal antibody (UM500070, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human placenta with mouse anti-EGFR clone UMAB95 1:200 dilution of 400ug/mL using HIER citrate pH6.o pressure chamber. Placenta known to express high levels in the cytoplasm and membrane trophoblastic cells

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human lung cancer with mouse anti-EGFR clone UMAB95 1:200 dilution of 400ug/mL using HIER citrate pH6.0 pressure chamber. Expression in lung cancer cells are cytoplasmic and membraneous

Validation comment

IHC

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-EGFR mouse monoclonal antibody (UM500070). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Validation comment

10K-CHIP