Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1280 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse EGFR Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse EGFR in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) EGFR and less than 5% cross-reactivity with rhErbB2, rhErbB3, and rhErbB4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9EP98
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Epidermal growth factor receptor promotes cerebral and retinal invasion by Toxoplasma gondii.
Nanobody Targeting of Epidermal Growth Factor Receptor (EGFR) Ectodomain Variants Overcomes Resistance to Therapeutic EGFR Antibodies.
The Immunogenicity and Anti-Tumor Efficacy of a Rationally Designed EGFR Vaccine.
iRhom2 promotes lupus nephritis through TNF-α and EGFR signaling.
Endocannabinoid Signaling in Embryonic Neuronal Motility and Cell-Cell Contact - Role of mGluR5 and TRPC3 Channels.
EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons.
T cells enhance stem-like properties and conditional malignancy in gliomas.
Corcino YL, Portillo JC, Subauste CS
Scientific reports 2019 Jan 24;9(1):669
Scientific reports 2019 Jan 24;9(1):669
Nanobody Targeting of Epidermal Growth Factor Receptor (EGFR) Ectodomain Variants Overcomes Resistance to Therapeutic EGFR Antibodies.
Tintelnot J, Baum N, Schultheiß C, Braig F, Trentmann M, Finter J, Fumey W, Bannas P, Fehse B, Riecken K, Schuetze K, Bokemeyer C, Rösner T, Valerius T, Peipp M, Koch-Nolte F, Binder M
Molecular cancer therapeutics 2019 Apr;18(4):823-833
Molecular cancer therapeutics 2019 Apr;18(4):823-833
The Immunogenicity and Anti-Tumor Efficacy of a Rationally Designed EGFR Vaccine.
Cheng C, Deng L, Li R
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(1):46-56
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(1):46-56
iRhom2 promotes lupus nephritis through TNF-α and EGFR signaling.
Qing X, Chinenov Y, Redecha P, Madaio M, Roelofs JJ, Farber G, Issuree PD, Donlin L, Mcllwain DR, Mak TW, Blobel CP, Salmon JE
The Journal of clinical investigation 2018 Apr 2;128(4):1397-1412
The Journal of clinical investigation 2018 Apr 2;128(4):1397-1412
Endocannabinoid Signaling in Embryonic Neuronal Motility and Cell-Cell Contact - Role of mGluR5 and TRPC3 Channels.
Turunen PM, Louhivuori LM, Louhivuori V, Kukkonen JP, Åkerman KE
Neuroscience 2018 Apr 1;375:135-148
Neuroscience 2018 Apr 1;375:135-148
EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons.
Puehringer D, Orel N, Lüningschrör P, Subramanian N, Herrmann T, Chao MV, Sendtner M
Nature neuroscience 2013 Apr;16(4):407-15
Nature neuroscience 2013 Apr;16(4):407-15
T cells enhance stem-like properties and conditional malignancy in gliomas.
Irvin DK, Jouanneau E, Duvall G, Zhang XX, Zhai Y, Sarayba D, Seksenyan A, Panwar A, Black KL, Wheeler CJ
PloS one 2010 Jun 7;5(6):e10974
PloS one 2010 Jun 7;5(6):e10974
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human EGFR by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EGFR at approximately 180-191 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human EGFR by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EGFR at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EGFR in Mouse Embryo. EGFR was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1280) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.