Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB8130 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-EGFR (Y869) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human Phospho-EGFR (Y869) in ELISA.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 869111
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-EGFR (Y869) by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-EGFR (Y869) Monoclonal Antibody (Catalog # MAB8130) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-EGFR (Y869) at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Phospho-EGFR (Y869) in A431 Human Cell Line. EGFR phosphorylated at Y869 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with Recombinant Human EGF (Catalog # 236-EG) using Rat Anti-Human Phospho-EGFR (Y869) Monoclonal Antibody (Catalog # MAB8130) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane upon stimulation. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.