Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- F48475 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- p-EGFR Antibody (pY1069)
- Antibody type
- Polyclonal
- Antigen
- This p-EGFR antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1069 of human EGFR.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the p-EGFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of EGFR in HeLa cell lysate, either noninduced (Lane 1) or induced with EGF (2).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from A431 cells,untreated or treated with EGF, using p-EGFR antibody (left) or nonphos Ab (right).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Dot blot analysis of p-EGFR antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted. P-Peptide=phos-peptide; NP-Peptide=nonphos-peptide.
