Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- F48552 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Phospho-EGFR Antibody (pY1016)
- Antibody type
- Polyclonal
- Antigen
- This phospho-EGFR antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1016 of human EGFR.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the phospho-EGFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from A431 cells (left to right), untreated or treated with EGF at 100ng/ml, using phospho-EGFR antibody (pY1016) or nonphos Ab at 1:8000 dilution.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Dot blot analysis of phospho-EGFR antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.