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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F49183 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- EGFR Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1004-1033 from the human protein was used as the immunogen for this anti-EGFR antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the anti-EGFR antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from A431, HUVEC, PC3 cell line (left to right) using anti-EGFR antibody diluted at 1:1000 for each lane.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Anti-EGFR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Anti-EGFR antibody flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
