AM00031PU-N

antibody from OriGene

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AM00031PU-N

Provider

OriGene

Product name

EGFR pTyr869 (incl. pos. control) mouse monoclonal antibody, clone 12A3, Purified

Antibody type

Monoclonal

Description

EGFR pTyr869 (incl. pos. control) mouse monoclonal antibody, clone 12A3, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

EGFR

Isotype

IgG

Antibody clone number

12A3

Vial size

100 µg

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-12A3 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).

Validation comment

WB