NBP2-52699-0.2mg
antibody from Novus Biologicals
Targeting: EGFR
ERBB, ERBB1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-52699-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal EGFR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Recognizes the extracellular domain of EGFR.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: EGFR Antibody (528) [NBP2-52699] - Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 (NBP2-52651) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. NBP2-52651 successfully detected the canonical human DC-SIGNR, as well as multiple other isoforms.