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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- 50-9509-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EGFR Monoclonal Antibody (me1B3), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This me1B3 monoclonal antibody reacts with human epidermal growth factor receptor (EGFR), also known as ErbB1 and Her1. EGFR is a receptor tyrosine kinase belonging to the EGFR/ErbB receptor subfamily, which also includes ErbB2/Her2/neu. EGFR contains an extracellular ligand-binding domain, a single transmembrane-spanning region, and a cytoplasmic tail containing a tyrosine kinase domain. Specific ligands include EGF and TNF alpha, as well as other members of the EGF protein family. Upon ligand binding, EGFR undergoes receptor-mediated dimerization with itself or ErbB2/Her2/neu. Dimerization induces auto-phosphorylation, resulting in the activation of PLC gamma, JAK/STAT, MAPK/ERK, and PI3K/AKT signaling pathways. EGFR has a role in cell proliferation, migration and differentiation, and is evolutionarily highly conserved in structure and function. EGFR is broadly expressed and is overexpressed or mutated in glioblastomas and a number of different epithelial cancers, including non-small cell lung cancer and colorectal cancer.
- Antibody clone number
- me1B3
- Concentration
- 5 µL/Test
Submitted references Oncogenic mutations counteract intrinsic disorder in the EGFR kinase and promote receptor dimerization.
Cell signaling by receptor tyrosine kinases.
Crystal structure of human epidermal growth factor and its dimerization.
Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin.
Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells.
Shan Y, Eastwood MP, Zhang X, Kim ET, Arkhipov A, Dror RO, Jumper J, Kuriyan J, Shaw DE
Cell 2012 May 11;149(4):860-70
Cell 2012 May 11;149(4):860-70
Cell signaling by receptor tyrosine kinases.
Lemmon MA, Schlessinger J
Cell 2010 Jun 25;141(7):1117-34
Cell 2010 Jun 25;141(7):1117-34
Crystal structure of human epidermal growth factor and its dimerization.
Lu HS, Chai JJ, Li M, Huang BR, He CH, Bi RC
The Journal of biological chemistry 2001 Sep 14;276(37):34913-7
The Journal of biological chemistry 2001 Sep 14;276(37):34913-7
Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin.
Libermann TA, Nusbaum HR, Razon N, Kris R, Lax I, Soreq H, Whittle N, Waterfield MD, Ullrich A, Schlessinger J
Nature 1985 Jan 10-18;313(5998):144-7
Nature 1985 Jan 10-18;313(5998):144-7
Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells.
Merlino GT, Xu YH, Ishii S, Clark AJ, Semba K, Toyoshima K, Yamamoto T, Pastan I
Science (New York, N.Y.) 1984 Apr 27;224(4647):417-9
Science (New York, N.Y.) 1984 Apr 27;224(4647):417-9
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of HeLa cells with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (blue histogram) or Anti-Human Epidermal Growth Factor Receptor (EGFR) eFluor® 660 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.
