Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA7-00308-A555 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48), Alexa Fluor™ 555
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with canine, mouse and rat based on sequence homology.
- Antibody clone number
- 30H45L48
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EGFR (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a EGFR Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA7-00308-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.