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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 710123 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-EGFR (Tyr1068) Recombinant Polyclonal Antibody (2HCLC)
- Antibody type
- Other
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat, non-human primate and Drosophila based on sequence homology. Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 2HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Phospho-EGFR pTyr1068 in whole cell extracts of A431 cells (lane 1 and 2) and NIH-3T3 cells (lane 3 and 4) treated with EGF (100 ng/mL, 5 min) using a Phospho-EGFR pTyr1068 Recombinant Rabbit Polyclonal Antibody (Product # 710123) at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lanes 2 and 4). Results show a band at ~175 kDa.
