LS-C788122

antibody from LSBio

Targeting: EGFR ERBB, ERBB1

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C788122

Provider

LSBio

Product name

EGFR Antibody (clone OTI3H2, Carrier-free) LS-C788122

Antibody type

Monoclonal

Description

Affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI3H2

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGFR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGFR.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-EGFR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).