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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [5]
- Immunoprecipitation [3]
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Validation data
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- Product number
- LS-C812958 - Provider product page
- Provider
- LSBio
- Product name
- EGFR Antibody LS-C812958
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot detection of EGFR in A549 and MDA-MB-468 cell lysates using EGFR mouse mAb (dilution 1:1000). Predicted band size: 134 Kda.Observed band size: 175KDa.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot detection of EGFR in A549 and COS7 cell lysates using EGFR mouse mAb (dilution 1:2000). Predicted band size: 134 Kda. Observed band size: 175KDa.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot detection of EGFR in A549 and COS7 cell lysates using EGFR mouse mAb (dilution 1:2000). Predicted band size: 134 Kda. Observed band size: 175KDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.
