Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 352908 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#352908, RRID:AB_11126165
- Product name
- Alexa Fluor® 488 anti-human EGFR [AY13]
- Antibody type
- Monoclonal
- Antigen
- Non-small cell lung cancer (NSCLC) cell line NCI-H322
- Description
- Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Human cervical cancer cell line HELA was stained with EGFR (clone AY13) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
- Conjugate
- Green dye
