MA5-15199

antibody from Invitrogen Antibodies

Targeting: EGFR ERBB, ERBB1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-15199

Provider

Invitrogen Antibodies

Product name

Phospho-EGFR (Tyr1068) Monoclonal Antibody (S.684.2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S.684.2

Vial size

100 µL

Concentration

1865 µg/mL

Storage

-20°C

References

Submitted references

Quercetin Reverses Rat Liver Preneoplastic Lesions Induced by Chemical Carcinogenesis.

Carrasco-Torres G, Monroy-Ramírez HC, Martínez-Guerra AA, Baltiérrez-Hoyos R, Romero-Tlalolini MLÁ, Villa-Treviño S, Sánchez-Chino X, Vásquez-Garzón VR
Oxidative medicine and cellular longevity 2017;2017:4674918

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Phospho-EGFR (Tyr1068) was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-EGFR (Tyr1068) Mouse Monoclonal Antibody (S.684.2) (Product # MA5-15199) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labelled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:100). Panel d represents the merged image showing membrane localization. Panel e represents cells treated with antagonist, Afatinib (1µM for 6hrs) followed by EGF (200 ng/mL for 10 minutes), showing no signal. Panel f shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.