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Immunocytochemistry
Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 1956-MSM1-P0 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EGFR (Epidermal Growth Factor Receptor) Monoclonal Antibody (GFR450)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GFR450
- Vial size
- 20 µg
- Concentration
- 200 µg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal Immunofluorescent analysis of A431 cells using AF488-labeled Isotype Control MAb (IgG2a) (Green). DAPI was used to stain the cell nuclei (blue). (Negative Control).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal Immunofluorescent analysis of A431 cells using AF488-labeled EGFR Monoclonal Antibody (GFR450) (Green). DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of human EGFR on A431 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled EGFR Mouse Monoclonal Antibody (GFR450).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Mouse Monoclonal Antibody (GFR/450) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the diFerence (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SDS-PAGE analysis Purified EGFR Mouse Monoclonal Antibody (GFR/450). Confirmation of Integrity and Purity of Antibody.
