Antibody data

Product number
Ab101163 - Provider product page
Provider
Aladdin Scientific
Product name
EGFR Mouse mAb
Antibody type
Monoclonal
Description
Mouse anti Human EGFR Antibody, Monoclonal(C4), could be used for WB, IHC and so on .Application:WB: 0.01-2 µg/mLIHC: 5-20 µg/mLProtein FunctionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.Isoform 2 may act as an antagonist of EGF action.
Reactivity
Human, Mouse
Host
Mouse
Conjugate
Unconjugated
Antigen sequence
AA 888-1210
Isotype
IgG
Antibody clone number
C4
Vial size
100_l,10_l,1ml,20_l,50_l
Concentration
Lot by Lot
Storage
Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
EGFR protein structure - Ab101163 shown in red.