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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP1-44893 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-44893, RRID:AB_10008113
- Product name
- Mouse Monoclonal EGFR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody EM-13 reacts with human EGFR (ErbB1/ HER1) phosphorylated on tyrosine 1173.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Acquired resistance to BRAF inhibition in BRAFV600E mutant gliomas.
Yao TW, Zhang J, Prados M, Weiss WA, James CD, Nicolaides T
Oncotarget 2017 Jan 3;8(1):583-595
Oncotarget 2017 Jan 3;8(1):583-595
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - Analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: EGF R/ErbB1 [p Tyr1173] Antibody (EM-13) [NBP1-44893] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.
