MA5-13343

antibody from Invitrogen Antibodies

Targeting: EGFR ERBB, ERBB1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13343

Provider

Invitrogen Antibodies

Product name

Anti-EGFR Antibody Cocktail

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-13356 targets Paxillin in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples. The MA5-13356 immunogen is purified recombinant full-length human paxillin protein.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

111.6, 199.12, F4, 11E8

Vial size

500 µL

Concentration

0.2 mg/ml

Storage

4° C

References

Submitted references

Cancer cells exploit eIF4E2-directed synthesis of hypoxia response proteins to drive tumor progression.

Uniacke J, Perera JK, Lachance G, Francisco CB, Lee S
Cancer research 2014 Mar 1;74(5):1379-89

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Metastasis suppressor tetraspanin CD82/KAI1 regulates ubiquitylation of epidermal growth factor receptor.

Odintsova E, van Niel G, Conjeaud H, Raposo G, Iwamoto R, Mekada E, Berditchevski F
The Journal of biological chemistry 2013 Sep 6;288(36):26323-34

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Facile chemical functionalization of proteins through intein-linked yeast display.

Marshall CJ, Agarwal N, Kalia J, Grosskopf VA, McGrath NA, Abbott NL, Raines RT, Shusta EV
Bioconjugate chemistry 2013 Sep 18;24(9):1634-44

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Regulation of EGFR trafficking and cell signaling by Sprouty2 and MIG6 in lung cancer cells.

Walsh AM, Lazzara MJ
Journal of cell science 2013 Oct 1;126(Pt 19):4339-48

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An oxygen-regulated switch in the protein synthesis machinery.

Uniacke J, Holterman CE, Lachance G, Franovic A, Jacob MD, Fabian MR, Payette J, Holcik M, Pause A, Lee S
Nature 2012 May 6;486(7401):126-9

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Ubiquitin-dependent regulation of COPII coat size and function.

Jin L, Pahuja KB, Wickliffe KE, Gorur A, Baumgärtel C, Schekman R, Rape M
Nature 2012 Feb 22;482(7386):495-500

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Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.

Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK
Molecular & cellular proteomics : MCP 2010 Jun;9(6):1085-99

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Structure-function analysis of nucleolin and ErbB receptors interactions.

Farin K, Di Segni A, Mor A, Pinkas-Kramarski R
PloS one 2009 Jul 3;4(7):e6128

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MMTV-EGF receptor transgene promotes preneoplastic conversion of multiple steroid hormone-responsive tissues.

Marozkina NV, Stiefel SM, Frierson HF Jr, Parsons SJ
Journal of cellular biochemistry 2008 Apr 15;103(6):2010-8

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Epidermal growth factor ligand/receptor loop and downstream signaling activation pattern in completely resected nonsmall cell lung cancer.

Volante M, Saviozzi S, Rapa I, Ceppi P, Cappia S, Calogero R, Novello S, Borasio P, Papotti M, Scagliotti GV
Cancer 2007 Sep 15;110(6):1321-8

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Sulindac metabolites inhibit epidermal growth factor receptor activation and expression.

Pangburn HA, Kraus H, Ahnen DJ, Rice PL
Journal of carcinogenesis 2005 Sep 2;4:16

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Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex.

Xu W, Yuan X, Xiang Z, Mimnaugh E, Marcu M, Neckers L
Nature structural & molecular biology 2005 Feb;12(2):120-6

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Autocrine extracellular signal-regulated kinase (ERK) activation in normal human keratinocytes: metalloproteinase-mediated release of amphiregulin triggers signaling from ErbB1 to ERK.

Kansra S, Stoll SW, Johnson JL, Elder JT
Molecular biology of the cell 2004 Sep;15(9):4299-309

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In vitro chemoresistance and biomarker profiles are unique for histologic subtypes of epithelial ovarian cancer.

Cloven NG, Kyshtoobayeva A, Burger RA, Yu IR, Fruehauf JP
Gynecologic oncology 2004 Jan;92(1):160-6

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Characterization of a transplantable hormone-responsive human prostatic cancer xenograft TEN12 and its androgen-resistant sublines.

Harper ME, Goddard L, Smith C, Nicholson RI
The Prostate 2004 Jan 1;58(1):13-22

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Racial disparity of epidermal growth factor receptor expression in prostate cancer.

Shuch B, Mikhail M, Satagopan J, Lee P, Yee H, Chang C, Cordon-Cardo C, Taneja SS, Osman I
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2004 Dec 1;22(23):4725-9

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Neuregulin expression, function, and signaling in human ovarian cancer cells.

Gilmour LM, Macleod KG, McCaig A, Sewell JM, Gullick WJ, Smyth JF, Langdon SP
Clinical cancer research : an official journal of the American Association for Cancer Research 2002 Dec;8(12):3933-42

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Differential cytoskeletal association of ErbB1 and ErbB2 during keratinocyte differentiation.

Kansra S, Stoll SW, Elder JT
Biochemical and biophysical research communications 2002 Aug 2;295(5):1108-17

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of EGFR was done on 90% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled EGFR (111.6+199.12+ F4+11E8) Mouse Monoclonal Antibody (Product # MA5-13343) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of EGFR was done on A-431 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with EGFR Mouse Monoclonal Antibody (MA513343, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Epidermal Growth Factor Receptor using Epidermal Growth Factor Receptor Monoclonal Antibody (Product # MA5-13343) on Native Human A431 Cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP-MS enrichment of EGFR (LFQ intensity): EGFR was enriched 586-fold from A549 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and EGFR antibody (Product # MA5-13343). See more information on IP-MS verification of antibody selectivity.