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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- AM39000PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM39000PU-N, RRID:AB_10976486
- Product name
- anti EGFR / ERBB1
- Antibody type
- Monoclonal
- Antigen
- Recombinant human EGFR (424-605aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT2H8
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- The extracts of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: The extracts of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. Monoclonal anti-human EGFR antibody (1:250) was stained with alexa 488 (green).
