AM00031PU-N

antibody from Acris Antibodies GmbH

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AM00031PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00031PU-N, RRID:AB_980943

Product name

anti EGFR / ERBB1 pTyr869 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Phosphopeptide conjugated to KLHEpitope: Phosphotyrosine 869

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

12A3

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Phosphospecificity: Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) HepG2 and A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-12A3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).