AM00047PU-N

antibody from Acris Antibodies GmbH

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AM00047PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00047PU-N, RRID:AB_980918

Product name

anti EGFR / ERBB1 (C-term) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Peptide conjugated to KLH. Epitope: C-terminus (aa 1165-1186) independent of phosphorylation status.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

13G8

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous EGFR:Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-13G8 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: A549; lane 3: SKOV3; lane 4: OVCAR5; lane 5: HaCaT; lane 6: PC3; lane 7: HeLa; lane 8: HepG2