Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-2731 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2731, RRID:AB_10148320
- Product name
- Mouse Monoclonal EGFR Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
The epitope is aa 985-996 in the cy
toplasmic domain of human EGFR. Rea
cts with both phosphorylated and un
phosphorylated EGFR.- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Frozen: EGF R/ErbB1 Antibody (F4) [NB100-2731] - IHC-Frozen analysis of a cryosection of human squamous cell carcinoma (vulva) using EGF R/ErbB1 antibody (clone F4). The signal was detected using alkaline phosphatase - anti-alkaline phosphatase (APAAP) method with red dye (Fast Red TR). The nuclei were counterstained using hematoxylin. This antibody generated specific staining of EGFR in the membranes of the cancer cells.