AM20237PU-S

antibody from Acris Antibodies GmbH

Targeting: EGFR ERBB, ERBB1

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20237PU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20237PU-S, RRID:AB_10755331

Product name

anti EGFR / ERBB1 (Extracell. non Ligand binding Site)

Antibody type

Monoclonal

Antigen

Human EGF-Receptor

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

EGF-R2

Vial size

10 µg

Immunoelectron microscopy

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Electron Microscopy Image of internalized EGF-R antibody complexes in A431 cells. The cells were stained with AM20237PU-N/AM20237PU-S for 1h at 4°C, followd by Peroxidase-conjugated Goat anti-Mouse IgG. After fixation with 2.5% glutaraldehyde, the signal was detected using DAB (0.6 mg/ml) for 20 min at RT. The cells were then processed for the preparation of ultrathin sections. Reins HA at al. (1993) J. Cell. Biochem 51 (2): 236-48.