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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-1884 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1884, RRID:AB_2231197
- Product name
- Goat Polyclonal SPFH2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoform 1 (NP_009106) only.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Membrane-associated ubiquitin ligase complex containing gp78 mediates sterol-accelerated degradation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
SPFH2 mediates the endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors and other substrates in mammalian cells.
A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes.
Jo Y, Sguigna PV, DeBose-Boyd RA
The Journal of biological chemistry 2011 Apr 29;286(17):15022-31
The Journal of biological chemistry 2011 Apr 29;286(17):15022-31
SPFH2 mediates the endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors and other substrates in mammalian cells.
Pearce MM, Wang Y, Kelley GG, Wojcikiewicz RJ
The Journal of biological chemistry 2007 Jul 13;282(28):20104-15
The Journal of biological chemistry 2007 Jul 13;282(28):20104-15
A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes.
Garcia MJ, Pole JC, Chin SF, Teschendorff A, Naderi A, Ozdag H, Vias M, Kranjac T, Subkhankulova T, Paish C, Ellis I, Brenton JD, Edwards PA, Caldas C
Oncogene 2005 Aug 4;24(33):5235-45
Oncogene 2005 Aug 4;24(33):5235-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPFH2 Antibody [NB100-1884] - Analysis of SPFH2 on Human Breast lysate using this antibody at 1 ug/ml (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
