PA5-71202

antibody from Invitrogen Antibodies

Targeting: OR7A17 HTPCRX19

Provider product page for PA5-71202

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Other assay [3]

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Product number: PA5-71202 - Provider product page
Provider: Invitrogen Antibodies
Product name: OR7A17 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This target displays homology in the following species: Cow: 86%; Dog: 79%; Guinea Pig: 79%; Horse: 86%; Human: 100%; Mouse: 79%; Pig: 86%; Rabbit: 79%; Rat: 86%
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

OR7A17 protein structure - PA5-71202 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 All- trans retinoic acid downregulates the expression of OR7A17. ( A , B ) The expression of OR7A17 in HaCaT cells was determined via reverse transcription quantitative PCR (RT-qPCR) ( A ) and Western blot analysis ( B ). In addition, OR7A17 expression was determined in human dermal fibroblasts and human epidermal melanocytes ( B ). HDFa: human dermal fibroblasts, HEMn: human epidermal melanocytes. ( C ) After ATRA treatment for 24 h, the cells were lysed and subjected to OR7A17 luciferase reporter assays. ( D ) Changes in the mRNA levels of OR7A17 in HaCaT cells were observed following treatment with ATRA for 24 h. ( E ) Changes in OR7A17 protein levels in HaCaT cells treated with ATRA for 24 h were determined via Western blot analysis. Data are presented as the mean +- SEM of at least three independent experiments. The statistical significance of differences between groups was evaluated via one-way analysis of variance (ANOVA), followed by Tukey's multiple-comparison test, using the GraphPad Prism 5 software. * p < 0.05 vs. control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 ATRA-induced downregulation of OR7A17 expression is mediated via RAR signaling. ( A ) A putative RARE sequence in the promoter region of the OR7A17 gene. ( B , C ) HaCaT cells were treated with 2 uM of ATRA and either 10 uM of RAR alpha antagonist ( B ) RO 41-5253 or 2 uM of RAR gamma antagonist ( C ) MM11253 for 24 h. After 24 h of incubation, the cells were lysed, and Western blotting was performed to determine the protein levels of OR7A17. ( D ) The recovery of HaCaT proliferation following treatment with RO 41-5253/MM11253 and ATRA was observed via EdU incorporation assays. Data are presented as the mean +- SEM of more than three independent experiments. The statistical significance of differences between groups was evaluated via one-way analysis of variance (ANOVA), followed by Tukey's multiple-comparison test, using the GraphPad Prism 5 software. * p < 0.05 vs. control, # p < 0.05 vs. ATRA-treated control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Overexpression of OR7A17 interferes with the suppressive effect of ATRA on Ca 2+ influx. ( A ) HaCaT cells were transduced with either LvCMV-GFP (mock-transduced HaCaT cell line) or LvCMV-OR7A17 (HaCaT-OR7A17 stable cell line). The overexpression of OR7A17 in HaCaT-OR7A17 cells was validated via Western blot analysis. ( B ) HaCaT-OR7A17 cells and mock-transduced HaCaT cells were treated with ATRA for 24 h, and OR7A17 expression was determined via Western blot analysis. Data are presented as the mean +- SEM of more than three independent experiments. * p < 0.05 vs. control. Changes in Ca 2+ influx after ATRA treatment were observed in ( C ) mock-transduced HaCaT cells and ( D ) HaCaT-OR7A17 cells. Cells were incubated with Fluo-4 NW Ca 2+ reagent loading solution for 30 min at 37 degC and for another 30 min at room temperature. Fluorescence was measured immediately after ATRA treatment. The data presented are representative of at least three independent experiments. ( E ) Both mock-transduced HaCaT cells and HaCaT-OR7A17 cells were treated with ATRA and incubated for 24 h in an incubator at 5% CO 2 and 37 degC. After 24 h of incubation, Fluo-4 NW Ca 2+ reagent loading solution was added to the cells and they were incubated for 30 min at 37 degC and another 30 min at room temperature. Fluorescence was measured after the incubation. ( F ) Changes in Ca 2+ influx after ethylenediaminetetraacetic acid (EDTA) treatment were observed in mock-transduced HaCaT cells. Cells we