PA5-23086

antibody from Invitrogen Antibodies

Targeting: LAMTOR1 C11orf59, FLJ20625, p18, p27RF-Rho, Pdro, Ragulator1

Western blot

Antibody data

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Validation data

Product number: PA5-23086 - Provider product page
Provider: Invitrogen Antibodies
Product name: LAMTOR1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: The target sequence has 98% sequence homology with bovine.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 50 µL
Concentration: 0.2 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

LAMTOR1 protein structure - PA5-23086 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of LAMTOR1 using a polyclonal antibody (Product # PA5-23086).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of LAMTOR1 in HepG2 cells. Samples were incubated in LAMTOR1 polyclonal antibody (Product # PA5-23086) using a dilution of 1:50 followed by FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Ragulator complex protein LAMTOR1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LAMTOR1 Polyclonal Antibody (Product # PA5-23086) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic along with golgi localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Ragulator complex protein LAMTOR1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LAMTOR1 Polyclonal Antibody (Product # PA5-23086) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic along with golgi localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.