Antibody data
- Antibody Data
 - Antigen structure
 - References [2]
 - Comments [0]
 - Validations
 - Western blot [2]
 - ELISA [1]
 - Immunocytochemistry [1]
 
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Validation data
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- Product number
 - H00009554-M01 - Provider product page

 - Provider
 - Abnova Corporation
 - Proper citation
 - Abnova Corporation Cat#H00009554-M01, RRID:AB_530195
 - Product name
 - SEC22L1 monoclonal antibody (M01), clone 1E1
 - Antibody type
 - Monoclonal
 - Description
 - Mouse monoclonal antibody raised against a partial recombinant SEC22L1.
 - Antigen sequence
 MVLLTMIARVADGLPLAASMQEDEQSGRDLQQYQS
QAKQLFRKLNEQSPTRCTLEAGAMTFHYIIEQGVC
YLVLCEAAFPKKLAFAYLEDLHSEFDEQHGKKVPT
VSRPY- Isotype
 - IgG
 - Antibody clone number
 - 1E1
 - Storage
 - Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
 
Submitted references		A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels.
				
Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells.
				
		
	
			Flowerdew SE, Burgoyne RD
The Biochemical journal 2009 Mar 15;418(3):529-40
		The Biochemical journal 2009 Mar 15;418(3):529-40
Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells.
			Tan HT, Tan S, Lin Q, Lim TK, Hew CL, Chung MC
Molecular & cellular proteomics : MCP 2008 Jun;7(6):1174-85
		Molecular & cellular proteomics : MCP 2008 Jun;7(6):1174-85
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					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - SEC22L1 monoclonal antibody (M01), clone 1E1 Western Blot analysis of SEC22L1 expression in HeLa ( Cat # L013V1 ).
 
- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - SEC22L1 monoclonal antibody (M01), clone 1E1. Western Blot analysis of SEC22L1 expression in PC-12 ( Cat # L012V1 ).
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Detection limit for recombinant GST tagged SEC22L1 is approximately 0.3ng/ml as a capture antibody.
 - Validation comment
 - Sandwich ELISA (Recombinant protein)
 - Protocol
 - Protocol
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence of monoclonal antibody to SEC22L1 on HeLa cell. [antibody concentration 10 ug/ml]
 - Validation comment
 - Immunofluorescence
 - Protocol
 - Protocol