MA5-50159

antibody from Invitrogen Antibodies

Targeting: SOCS7 NAP4, NCKAP4

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-50159

Provider

Invitrogen Antibodies

Product name

SOCS7 Recombinant Rabbit Monoclonal Antibody (23E4)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

23E4

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

-20°C or -80°C if preferred

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis with a SOCS7 monoclonal antibody (Product # MA5-50159) using a dilution of 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.43% DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis overlay peak curve showing HepG2 cells stained with SOCS7 monoclonal antibody (Product # MA5-50159) for 45 min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at (1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.