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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX118549 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX118549, RRID:AB_11164506
- Product name
- Syntaxin 3 antibody [N1C2]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HeLa B: HCT116 12% SDS PAGE GTX118549 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntaxin 3 antibody [N1C2] (GTX118549) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Syntaxin 3 antibody [N1C2] detects Syntaxin 3 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: Syntaxin 3 protein stained by Syntaxin 3 antibody [N1C2] (GTX118549) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
