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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX122467 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX122467, RRID:AB_11164984
- Product name
- CYFIP1 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Co-regulation of mRNA translation by TDP-43 and Fragile X Syndrome protein FMRP.
Majumder P, Chu JF, Chatterjee B, Swamy KB, Shen CJ
Acta neuropathologica 2016 Nov;132(5):721-738
Acta neuropathologica 2016 Nov;132(5):721-738
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 5% SDS PAGE GTX122467 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: BCL-1 5% SDS PAGE GTX122467 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with CYFIP1 antibody [N1N2], N-term (GTX122467) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using CYFIP1(GTX122467) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CYFIP1 antibody [N1N2], N-term detects CYFIP1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CYFIP1 stained by CYFIP1 antibody [N1N2], N-term (GTX122467) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of CYFIP1 protein from HeLa whole cell extracts using 5 £gg of CYFIP1 antibody [N1N2], N-term (GTX122467).Western blot analysis was performed using CYFIP1 antibody [N1N2], N-term (GTX122467).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using CYFIP1(GTX122467) antibody at 1:500 dilution.
